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The Fast Module for ZEISS LSM 880 with Airyscan: Confocal Superresolution Imaging with Four Times the Speed and Improved Signal-to-Noise Ratio

First introduced in August 2014, the Airyscan detector from ZEISS represents a new detector concept for laser scanning microscopy (LSM) that enables a simultaneous resolution and signal-to-noise (SNR) increase over traditional LSM imaging. The Airyscan detector design substitutes the conventional LSM detector and pinhole scheme for an array of 32 sensitive GaAsP detector elements, arranged […]

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In Microscopy & Imaging 31st of May, 2016 Presented by: Chris Power
Article

The World of Microbes Part 1: Antibiotics and Vaccines

The world as we know it couldn’t exist without microbes. It is estimated that there are tens of trillions of bacteria living just in and on humans. (Ewww?) One trillion has 12 zeros. To put that in...
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Article

Defeat RNAse Contamination Using Bleach in Your RNA Agarose Gel

So, you’ve extracted your precious RNA and want to check its quality on a gel. Conventionally, you would run a formaldehyde gel, which is messy and requires a lot of prep. Plus, it is a huge undertaking in...
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Article

Navigating the Sea of Human Genetics, Part I

Ahoy mateys! At my workplace we've recently become EXTREMELY excited about a resource called the HuGE Navigator. It's so great that it's going to take me a couple of articles to highlight its features. Hop on...
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Article

7 Helpful Tips to Get You Through Your First Grad School Week

It’s official. You’ve signed on the dotted line and you are about to begin the most exciting and frustrating journey into the depths of the unknown: a journey otherwise known as a PhD. You’ve heard the...
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Article

The Key to Unlocking DNA from FFPE Tissues

Formalin fixed paraffin embedded (FFPE) tissues are valuable samples that typically come from human specimens collected for examination of the histology of biopsies for the detection of cancer. But each...
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Article

Carrying You Through Gas Chromatography

Maybe you found a paper in which they used gas chromatography (GC) to analyze a sample that is similar to yours and now you are wondering if you should try it too. If you are hesitant because you know little...
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Article

Five Truths About Science Publishing or Why All Science Should be Free

After writing my last article, I have started illegally downloading hundreds of scientific publications from the pirate website, Sci-Hub. When I mentioned that at the last lab meeting, my labmates argued that...
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Article

Photonic PCR: When Lightening Strikes Your DNA

Before I get into today’s topic, please allow me to digress a bit and start with a few sentences that sum up the polymerase chain reaction (PCR); the grand-daddy of molecular biology. PCR, a method that is...
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Article

Sorting Large Cells and Materials by Flow Cytometry

Flow cytometers and cell sorters were designed with blood cells in mind. This means that commercial cell sorters are optimized for sorting cells typically smaller than about 20 µm in diameter. However, it...
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Article

Basic Care for Your Liquid Handler

Liquid handlers, such as the Eppendorf 5075 I use to prepare DNA libraries, can be an immense help in the lab. Not only can they provide “lights-out” automation, freeing you to do something else, but they...
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